After a brief training session, your examiners will have no trouble at all determining which of your samples contain human DNA and are suitable for further lab analysis. This short video shows just how quick and simple it can be to screen samples for DNA.
ParaDNA makes it quick and simple for you to triage DNA evidence collected from crime scenes and decide which samples are suitable for submission to the lab for conventional STR analysis.
It’s vital to work in a clean area and wear appropriate personal protective equipment (PPE). And clean down the work surface using standard laboratory cleaning material.
Each screen requires the use of a sample collector and screening test reaction plate. These are supplied in packs of eight independent screening tests.
The sample collector comes in a sterile sealed bag. Open the bag at the opposite end from the sampling 4-nib, taking care not to touch other objects with the 4-nib.
Slide the collar of the sample collector towards the sampling end until you hear it ‘click’. The four separate points of the 4-nib now become one to create a single sampling tip.
You can take samples either directly from the body fluid stain itself or from the evidence item, such as the top of a bottle.
Here, a blood stain is being sampled using a swab.
Place the swab head against a clean surface and brush it with the sample collector.
The ParaDNA Screening Test Reaction Plate is used to analyse the samples in the screening instrument within a closed system. It comes pre-loaded with all the chemistry needed to carry out the test.
A foil seal over the top of the plate prevents foreign material or DNA entering the reaction mix.
Remove the seal from the plate to reveal the wells where the 4-nib sample tip is to be inserted.
Carefully insert the 4-nib into the 4-wells of the plate and push down firmly until the rivet is fully inserted.
Snap off the sampling handle to create a sealed, closed reaction chamber.
You’re now ready to insert the plate into one of the four heads of the screening instrument.
The test is run and the whole operation managed using software installed on your PC. Selecting the head you want to use will enable you to unlock the lids.
Insert the sampled screening test reaction plate into the chosen head on the instrument. Its ‘key’ shape makes sure that you always position the plate in the correct way.
After you’ve closed the lid, click the green START icon on the PC screen and run the test.
After about 75 minutes, the results are displayed and you will know if you have a sample suitable for forensic lab analysis.
For each sample the result is displayed as red or green to provide a recommendation of whether to submit the sample or not.
ParaDNA not only collapses the DNA screening process from 24 hours to around 75 minutes, it enables you to progress only those samples that are relevant.
After 75 minutes, the system will tell you whether the sample screened will give a usable SGM Plus profile from follow up lab analysis. This means you can target the samples that are likely to deliver the most investigative leads.